KMID : 1130220170210020078
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Annals of Geriatric Medicine and Research 2017 Volume.21 No. 2 p.78 ~ p.85
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Effects of Homocysteine and Hyperglycemia on the Proliferation of Aortic Vascular Smooth Muscle Cells of Obese Type 2 Diabetes Rat
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Yoo Hyung-Joon
Yu Sung-Hoon Cho Young-Jung Nam Hong-Woo Kang Dong-Hyun
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Abstract
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Background: The aim of this study was to investigate the role of homocysteine on the proliferation of rat aortic vascular smooth muscle cells (VSMCs) by measuring mitogen-activated protein (MAP) kinase under hyperglycemic conditions.
Methods: Rat aortic VSMCs were isolated from Otsuka Long-Evans Tokushima Fatty and Long-Evans Tokushima Otsuka rats. VSMCs were incubated in the presence of homocysteine (1 mM) with/without PD98059 (30 ¥ìM) and wortmannin (300 nM) for 48 hours in different concentrations of glucose (5.5, 25 mM). Proliferation was evaluated by methylthiazoletetrazolium (MTT), fluorescence-activated cell sorting (FACS), and western blot analyses.
Results: In MTT and FACS analyses, the proliferation of VSMCs was increased by homocysteine. After PD98059 (30 ¥ìM) and wortmannin (300 nM) treatment with homocysteine (1 mM), the increased proliferation of VSMC caused by homocysteine, decreased to control levels. In Western blot analysis, immunoexpression of phospho-p44/42 MAP kinase was significantly increased by homocysteine (1 mM). After PD98059 and wortmannin treatment, the increased immunoexpression of phospho-p44/42 MAP kinase was suppressed.
Conclusion: These results suggest that the MAP kinase and PI3 kinase pathways are the main mechanisms involved in rat VSMC proliferation caused by homocysteine under hyperglycemic conditions.
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KEYWORD
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Homocysteine, Hyperglycemia, Vascular smooth muscle cell, Mitogen-activated protein kinase, Phosphoinositide 3-kinase
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